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A2220
Sigma
ANTI-FLAG® M2 Affinity Gel
purified immunoglobulin, buffered aqueous glycerol solution
| Synonym: | ANTI-FLAG® M2 Affinity Agarose Gel, Monoclonal ANTI-FLAG® M2 antibody produced in mouse |
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Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Application | Affinity Gel (Freezer safe) for the purification of FLAG fusion proteins. |
| General description | Anti-FLAG M2 binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent. |
| Other Notes | To view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature. |
| Specificity | Binds to the FLAG® epitope wherever it is located in the fusion protein: amino-terminal, Met-amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent. |
| Physical form | Suspension in buffered saline containing azide as preservative and 50% glycerol |
| Legal Information | ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| antibody form | purified immunoglobulin |
| form | buffered aqueous glycerol solution |
| isotype | IgG1 |
| capacity | >0.6 mg/mL, resin binding capacity (FLAG-BAP) |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 1 |
References
| reference | Brizzard, B.L., et al., Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques 16, 730-735, (1994) Abstract |
| Chiang CM, and Roeder RG., Expression and purification of general transcription factors by FLAG epitope-tagging and peptide elution Pept. Res. 6, 62-64, (1993) Abstract | |
| Knappik A, and Plückthun A., An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments Biotechniques 17, 754-761, (1994) Abstract | |
| Einhauer A, and Jungbauer A., The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins J. Biochem. Biophys. Methods 49, 455-465, (2001) Abstract |
