A2220

Sigma

 

ANTI-FLAG® M2 Affinity Gel

purified immunoglobulin, buffered aqueous glycerol solution

Synonym:ANTI-FLAG® M2 Affinity Agarose Gel, Monoclonal ANTI-FLAG® M2 antibody produced in mouse

Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
ApplicationAffinity Gel (Freezer safe) for the purification of FLAG fusion proteins.
General descriptionAnti-FLAG M2 binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.
Other NotesTo view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature.
SpecificityBinds to the FLAG® epitope wherever it is located in the fusion protein: amino-terminal, Met-amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent.
Physical formSuspension in buffered saline containing azide as preservative and 50% glycerol
Legal InformationANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
 FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody formpurified immunoglobulin
formbuffered aqueous glycerol solution
isotypeIgG1
capacity >0.6 mg/mL, resin binding capacity (FLAG-BAP)
shipped inwet ice
storage temp.−20°C

Safety

WGK Germany1

Related Products

Related productP8170, Protein G−Peroxidase from Streptococcus sp.
 4B200, Sepharose® 4B
 F1804, Monoclonal ANTI-FLAG® M2 antibody produced in mouse
 A0919, Mouse IgG−Agarose

References

referenceBrizzard, B.L., et al., Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution. Biotechniques 16, 730-735, (1994) Abstract
 Chiang CM, and Roeder RG., Expression and purification of general transcription factors by FLAG epitope-tagging and peptide elution Pept. Res. 6, 62-64, (1993) Abstract
 Knappik A, and Plückthun A., An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments Biotechniques 17, 754-761, (1994) Abstract
 Einhauer A, and Jungbauer A., The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins J. Biochem. Biophys. Methods 49, 455-465, (2001) Abstract